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Zebrafish
Facility
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The University
of Maine Zebrafish Facility is an outstanding asset for
students and researchers throughout the State and complements
research being conducted using other vertebrates such as mice.
This facility fosters scientific collaborations and interactions
with research institutions throughout the State.
Click
Here for more information about this state-of-the-art
facility
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DNA
Sequencing Service
ABI
377 Sequencer |
The University
of Maine DNA Sequencing Facility is equipped with an ABI model
373A Stretch DNA Sequencer with the XL Upgrade and an ABI
model 377 Sequencer with the XL upgrade. The sequencers are
each capable of running 64 samples per gel yielding 800-1,000
bases of sequence from a good DNA template. The turnaround
time per sample is usually 2-3 days depending upon the services
performed.
Click
here for more information about this service.
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Electron
Microscopy Facility
| UMaine's
Electron Microscopy Laboratory (EML) offers the tools and technical
expertise for research and training in microscopy with both
light and electron microscopes. The lab operates three electron
microscopes (one scanning and two transmission electron microscopes)
and the ancillary equipment needed for preparation of specimens
for EM and other microscopy. |
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The EML's variety
of light microscopes encompasses optics for bright-field,
phase-contrast, differential-interference, and fluorescence
imaging.
Click
Here for more information about our extensive EM facilities
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Confocal
Microscopy Facility
| The
Confocal Microscopy Facility at the University of Maine boasts
a Leica TCS SP laser scanning confocal microscope equipped with
hardware and software suitable for a wide range of biological
imaging applications. A research microscope is coupled to a
confocal three-laser scanning unit equipped with state-of-the-art
spectrometer detectors. In the confocal microscope, light from
structures in a specimen that are not in focus is supressed
at image formation, resulting in acquisition of an "optical
section". The image is obtained using an arrangement of pinholes
at conjugated points in the optical path. |
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The emitted/reflected
light is transformed by a photomultiplier detector into a
series of electrical signals which can then be stored digitally
and displayed on a computer monitor. The confocal microscope
allows the investigator to determine the relative location
of structures with high precision and to reconstruct an image
of the specimen in three dimensions.
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Digital
Imaging Facility
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This multi-user,
state-of-the-art imaging facility is equipped with a
selection of microscopes, cameras, and video equipment necessary
for
bioscience research. |
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Facility contains both upright and inverted wide-field fluorescence
microscopes, a Leica MZ FL fluorescence stereomicroscope, a
Sony digital video imaging system, bright field stereomicroscopes,
a SPOT2 cooled CCD color digital camera, and an Eppendorf Microinjection
System. |
Bioinformatics
Facility
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The
University of Maine Bioinformatics Facility offers hardware
and software for a variety of molecular biology and genomics
applications:
Hardware:
- (1) Sun Microsystems
360Mgz UltraSPARK 5 UNIX Workstation, 512 MB RAM, 8 MB
- (3) Apple MacIntosh
G4 dual processor (2 x 800Mgz), 512 MB RAM, 60GB
Software:
- Genetics Computer
Group (GCG) Wisconsin Package v. 10
- Genetics Computer
Group (GCG)
- SeqWeb v.1
- MacVector, Accelrys
Inc.
- Genetic Data
Environment (GDE)
- PHYLIP
- PAUP
- Dedicated Comparative
Sequence Editor (DCSE)
- RnaViz
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| Accounts
are available to University of Maine students, faculty, and
staff. Online account applications and information may be obtained
at: http://phoenix.umesci.maine.edu |
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Contact Us
The
Department of
Biochemistry, Microbiology and Molecular Biology
The University of Maine
5735 Hitchner Hall
Orono, Maine 04469-5735
Phone: 207-581-2810/2815
FAX: 207-581-2801
A
Member of the University of Maine System
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