THE ORDER MONOBLEPHARIDALES
OEDOGONIOMYCES IN THE CLASSROOM
CAUTION - Use sterile technique at all times. Sterilize all glassware and media by autoclaving before use.
LONG TERM MAINTENANCE – If desired, isolate CR90 of Oedogoniomyces is easily maintained in flasks containing 50 ml of T/2 stored at room temperature (22°C) and transferred every 3 months.
PREPARATION TIME - Begin “Day 0” 14 days before expected use.
DAY 0 - Shake stock flask, grown for at least 3 weeks, to loosen thalli and start new stock flask by transferring, with a sterile pipette, 1 or 2 mL from present stock to a new flask containing 50 mL of T/2. With sterile pipette, transfer 1 or more mL of the remaining material in the present stock flask to each sterilized Petri plate containing cover slips needed for class. Add enough sterile T/2 from stock bottle to cover the slips in each Petri plate. You will probably need to rearrange the cover slips with a sterile needle or other tool to spread them evenly over the bottom of the Petri plate. Grow for 2 weeks in the light at room temperature (~22° C).
IN CLASS - DAY 14 - Use sterile forceps to remove an individual cover slip, wipe the under side of the cover slip dry, and invert the cover slip over a small drop of water on a clean microscope slide for observation.
SCHEDULE ADJUSTMENT - If careful sterile technique is observed and the plates remain uncontaminated, observation should be possible for 2 or 3 additional days.
RECIPE - T/2 broth for flasks, stock bottle, and Petri dishes with cover slips.
MATERIALS NEEDED - In addition to 250 ml Erlenmyer flasks containing 50 ml of T/2 (for long term maintenance) you will need for each 5 students: 1 glass Petri dish containing 5 glass cover slips, thickness 1 ½ and 500 ml bottle for T/2 supply.
Emerson, Ralph and Howard C. Whisler. 1968. Cultural studies of Oedogoniomyces and Harpochytrium, and a proposal to place them in a new order of aquatic phycomycetes. Archiv für Mikrobiologie 61: 195 - 211.
Karling, John S.1977. Chytridiomycetarum Iconographia. Lubrecht & Cramer. Monticello,N.Y.
Kobayashi, Yoshio and Mariko Ôkubo. 1954. On new genus Oedogoniomyces of the Blastocladiaceae . Bulletin of the National Science Museum (Tokyo), N.S. Vol.1,No. 1(No.34) March, 1954.
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