Quick metod of preparation of members of the
Monoblepharidaceae and Gonapodyaceae for the classroom

            If sterile material is not needed for use in the classroom, members of Monoblepharidaceae and Gonapodyaceae can be prepared quickly and easily.  Grow the desired material on a sterile agar plate for about two weeks.  Using sterile technique cut the innoculant into small pieces and distribute it evenly about the plate so that several colonies result; grow until there is sufficient material for the class.

            When ready to use push aside the pieces of innoculant and cut the growing thalli into fine pieces.  Place the pieces of thallus in unsterilized pond water in two Petri dishes.  Keep one dish of each kind in the light and one in the dark for two to three days.  Both asexual and sexual growth will result.

            Cultures of Gonapodya polymorpha M# 36 respond to this treatment described above. Cultures of Gonapodya prolifera M# 6 usually do not respond but may be tried so students can compare sizes and growth habits.  Most cultures of Monoblepharis sps. also respond to this treatment.  Particularly useful are cultures M# 20F and M# 45E of Monoblephais polymorpha.  The response of culture M# 42 of Monoblepharis macrandra is prolific.  Some cultures of Monoblepharella produce zoosporangia by this method but require temperatures of 30° - 32° C to reproduce sexually. Culture M# 15 is most likely to be successful.  Also available are cultures of Gonapodya polymorpha, Gonapodya prolifera, Monoblepharis macrandra, and Monoblepharis polymorpha.

MATERIALS NEEDED - Sterile agar plates for cultures, glass Petri dishes, unsterilized pond water.


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