RECIPES

mPmTG agar for plates and slants (Longcore, 1992; modified from Barr, 1986)

0.4 g peptonized milk

0.4 g tryptone

2 g glucose

10 g agar for plates, 12 g for slants

1000 mL distilled water

T/2 broth for flasks and Petri dishes with cover slips (Fuller and Jaworski, 1987)

5 g tryptone

1000 mL distilled water

Lima bean agar is available from scientific supply companies but if exactness of ingredients is not important, it can be made in the lab more cheaply.

1000 mL distilled water

1 10 oz. package frozen lima beans from the grocery store

10 g agar for plates, 12 for slants

Boil the beans in the distilled water for ½ hr. Discard the beans and filter the liquid to remove as much of the resulting fibrous material as possible. Replace the evaporated distilled water, add the agar and mix well before and after autoclaving.

Lima bean liquid medium

1000 mL distilled water

1 10 oz. package frozen lima beans from the grocery store.

Boil the lima beans in the distilled water for ½ hour. Discard the beans and filter the liquid to remove as much of the resulting fibrous material as possible. Replace the evaporated distilled water, mix well before and after autoclaving, distribute to flasks and autoclave again.

1/3 PW - Collect water from a quiet, weedy area of the nearest small pond and dilute, 1 part pond water to 2 parts distilled water. Sterilize in bottles in autoclave. Keep unsterilized stock in refrigerator.